CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.1200 | Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A |
|
1.10.1200.10 | ACP-like |
Domain Context
CATH Clusters
| Superfamily | ACP-like |
| Functional Family | Isochorismatase |
Enzyme Information
| 6.3.2.14 |
Enterobactin synthase.
based on mapping to UniProt P0ADI4
6 ATP + 3 2,3-dihydroxybenzoate + 3 L-serine = enterobactin + 6 AMP + 6 diphosphate.
-!- This enzyme complex catalyzes the conversion of three molecules each of 2,3-dihydroxybenzoate and L-serine to form the siderophore enterobactin. -!- In Escherichia coli the complex is formed by EntB (an aryl carrier protein that has to be activated by 4'-phosphopantetheine), EntD (a phosphopantetheinyl transferase that activates EntB), EntE (catalyzes the ATP-dependent condensation of 2,3-dihydroxybenzoate and holo-EntB to form the covalently arylated form of EntB), and EntF (a four domain protein that catalyzes the activation of L-serine by ATP, the condensation of the activated L-serine with the activated 2,3- dihydroxybenzoate, and the trimerization of three such moieties to a single enterobactin molecule).
|
| 3.3.2.1 |
Isochorismatase.
based on mapping to UniProt P0ADI4
Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate.
|
UniProtKB Entries (1)
| P0ADI4 |
ENTB_ECOLI
Escherichia coli K-12
Enterobactin synthase component B
|
PDB Structure
| PDB | 2FQ1 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Structure of the EntB multidomain nonribosomal peptide synthetase and functional analysis of its interaction with the EntE adenylation domain.
Chem.Biol.
|