CATH Domain 2cgnA01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.120	Jelly Rolls
	2.60.120.10	Jelly Rolls

Domain Context

CATH Clusters

Superfamily	Jelly Rolls
Functional Family	Hypoxia-inducible factor 1-alpha inhibitor

Enzyme Information

1.14.11.n4

Ankyrin-repeat-histidine dioxagenase.

based on mapping to UniProt Q9NWT6

(1) [Ankyrin-repeat domain protein]-L-histidine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-histidine + succinate + CO(2). (2) [Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2). (3) [Ankyrin-repeat domain protein]-L-aspartate + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-aspartate + succinate + CO(2).

1.14.11.30

Hypoxia-inducible factor-asparagine dioxygenase.

based on mapping to UniProt Q9NWT6

Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia- inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2).

-!- Contains iron, and requires ascorbate. -!- Catalyzes hydroxylation of an asparagine in the C-terminal transcriptional activation domain of HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which reduces its interaction with the transcriptional coactivator protein p300. -!- The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.

UniProtKB Entries (1)

Q9NWT6

HIF1N_HUMAN

Homo sapiens

Hypoxia-inducible factor 1-alpha inhibitor

PDB Structure

PDB	2CGN
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural and Mechanistic Studies on the Inhibition of the Hypoxia-Inducible Transcription Factor Hydroxylases by Tricarboxylic Acid Cycle Intermediates. Hewitson, K., Lienard, B., McDonough, M.A., Clifton, I.J., Butler, D., Soares, A.S., Oldham, N.J., McNeill, L.A., Schofield, C.J. J.Biol.Chem.