CATH Domain 2bf8A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.10	Roll
	3.10.110	Ubiquitin Conjugating Enzyme
	3.10.110.10	Ubiquitin Conjugating Enzyme

Domain Context

CATH Clusters

Superfamily	Ubiquitin Conjugating Enzyme
Functional Family	Putative ubiquitin-conjugating enzyme e2 k

Enzyme Information

2.3.2.23

E2 ubiquitin-conjugating enzyme.

based on mapping to UniProt P61085

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.

-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (2)

P61085	UBE2K_BOVIN Bos taurus Ubiquitin-conjugating enzyme E2 K
P63165	SUMO1_HUMAN Homo sapiens Small ubiquitin-related modifier 1

PDB Structure

PDB	2BF8
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Sumo Imodification of the Ubiquitin Conjugating Enzyme E2-25K Pichler, A., Knipscheer, P., Oberhofer, E., Van Dijk, W.J., Korner, R., Velgaard Olsen, J., Jentsch, S., Melchior, F., Sixma, T.K. Nat.Struct.Mol.Biol.