CATH Domain 2am3A01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.550	Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
	3.90.550.10	Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Domain Context

CATH Clusters

Superfamily	Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A
Functional Family	Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Enzyme Information

2.4.1.101

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase.

based on mapping to UniProt P27115

UDP-N-acetyl-alpha-D-glucosamine + alpha-D-Man-(1->3)-[alpha-D-Man- (1->3)-(alpha-D-Man-(1->6))-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D- GlcNAc-(1->4)-alpha-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc- (1->2)-alpha-D-Man-(1->3)-[alpha-D-Man-(1->3)-(alpha-D-Man-(1->6))-alpha- D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-N- Asn-[protein].

-!- The enzyme, found in plants and animals, participates in the processing of N-glycans in the Golgi apparatus. -!- Its action is required before the other N-acetylglucosaminyltransferases involved in the process (GlcNAcT-II through VI) can act. -!- While the natural substrate (produced by EC 3.2.1.113) is described here, the minimal substrate recognized by the enzyme is alpha-D-Man- (1->3)-beta-D-Man-R. -!- Formerly EC 2.4.1.51.

UniProtKB Entries (1)

P27115

MGAT1_RABIT

Oryctolagus cuniculus

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

PDB Structure

PDB	2AM3
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Cricetulus
Primary Citation	X-ray Crystal Structures of Rabbit N-acetylglucosaminyltransferase I (GnT I) in Complex with Donor Substrate Analogues. Gordon, R.D., Sivarajah, P., Satkunarajah, M., Ma, D., Tarling, C.A., Vizitiu, D., Withers, S.G., Rini, J.M. J.Mol.Biol.