CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.90 | Alpha-Beta Complex |
|
3.90.730 | Ribonuclease Rh; Chain A |
|
3.90.730.10 | Ribonuclease T2-like |
Domain Context
CATH Clusters
| Superfamily | Ribonuclease T2-like |
| Functional Family |
Enzyme Information
| 4.6.1.19 |
Ribonuclease T(2).
based on mapping to UniProt P23540
RNA + H(2)O = an (RNA fragment)-3'-nucleoside-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment).
-!- A widely distributed family of related enzymes found in protozoans, plants, bacteria, animals and viruses that cleave ssRNA 3'-phosphate group with little base specificity. -!- The enzyme catalyzes a two-stage endonucleolytic cleavage. -!- The first reaction produces 5'-hydroxy-phosphooligonucletides and 3'-phosphooligonucleotides ending with a 2',3'-cyclic phosphodiester, which are released from the enzyme. -!- The enzyme then hydrolyzes the cyclic products in a second reaction that takes place only when all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The second reaction is a reversal of the first reaction using the hydroxyl group of water instead of the 5'-hydroxyl group of ribose. -!- The overall process is that of a phosphorus-oxygen lyase followed by hydrolysis to form the 3'-nucleotides. -!- Formerly EC 2.7.7.17, EC 3.1.4.23, and EC 3.1.27.1.
|
UniProtKB Entries (1)
| P23540 |
RNMC_MOMCH
Momordica charantia
Ribonuclease MC
|
PDB Structure
| PDB | 1UCC |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2'-UMP or 3'-UMP, reveal structural basis for uridine specificity
Biochem.Biophys.Res.Commun.
|
