CATH Domain 1nmtA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.630	Aminopeptidase
	3.40.630.30	Gcn5-related N-acetyltransferase (GNAT)

Domain Context

CATH Clusters

Superfamily	3.40.630.30
Functional Family	Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97

Glycylpeptide N-tetradecanoyltransferase.

based on mapping to UniProt P30418

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].

-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

P30418

NMT_CANAL

Candida albicans SC5314

Glycylpeptide N-tetradecanoyltransferase

PDB Structure

PDB	1NMT
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Crystal structure of the anti-fungal target N-myristoyl transferase. Weston, S.A., Camble, R., Colls, J., Rosenbrock, G., Taylor, I., Egerton, M., Tucker, A.D., Tunnicliffe, A., Mistry, A., Mancia, F., de la Fortelle, E., Irwin, J., Bricogne, G., Pauptit, R.A. Nat.Struct.Biol.