CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.720 | NAD(P)-binding Rossmann-like Domain |
Domain Context
CATH Clusters
| Superfamily | NAD(P)-binding Rossmann-like Domain |
| Functional Family | peroxisomal multifunctional enzyme type 2 |
Enzyme Information
| 4.2.1.119 |
Enoyl-CoA hydratase 2.
based on mapping to UniProt P97852
(3R)-3-hydroxyacyl-CoA = (2E)-2-enoyl-CoA + H(2)O.
-!- This enzyme catalyzes a hydration step in peroxisomal beta-oxidation. -!- The human multifunctional enzyme type 2 (MFE-2) is a 79000 Da enzyme composed of three functional units: (3R)-hydroxyacyl-CoA dehydrogenase, 2-enoyl-CoA hydratase 2 and sterol carrier protein 2-like units. -!- The enzymes from Aeromonas caviae and Arabidopsis thaliana are monofunctional enzymes. -!- 2-enoyl-CoA hydratase 3 from Candida tropicalis is a part from multifunctional enzyme type 2.
|
| 1.1.1.n12 |
(3R)-hydroxyacyl-CoA dehydrogenase.
based on mapping to UniProt P97852
(R)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
|
| 4.2.1.107 |
3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase.
based on mapping to UniProt P97852
(24R,25R)-3-alpha,7-alpha,12-alpha,24-tetrahydroxy-5-beta-cholestanoyl- CoA = (24E)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl- CoA + H(2)O.
-!- Forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. -!- Involved in the beta-oxidation of the cholesterol side chain in the cholic-acid biosynthesis pathway.
|
UniProtKB Entries (1)
| P97852 |
DHB4_RAT
Rattus norvegicus
Peroxisomal multifunctional enzyme type 2
|
PDB Structure
| PDB | 1GZ6 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Binary Structure of the Two-Domain (3R)-Hydroxyacyl-Coa Dehydrogenase from Rat Peroxisomal Multifunctional Enzyme Type 2 at 2.38 A Resolution
Structure
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