CATH Classification

Domain Context

CATH Clusters

Superfamily Toxin ADP-ribosyltransferase; Chain A, domain 1
Functional Family NAD(P)(+)--arginine ADP-ribosyltransferase

Enzyme Information

3.2.2.5
NAD(+) glycohydrolase.
based on mapping to UniProt P20974
NAD(+) + H(2)O = ADP-D-ribose + nicotinamide.
-!- This enzyme catalyzes the hydrolysis of NAD(+), without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). -!- The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. -!- The enzyme from the venom of the snake Agkistrodon acutus also catalyzes EC 3.6.1.5.
2.4.2.31
NAD(+)--protein-arginine ADP-ribosyltransferase.
based on mapping to UniProt P20974
NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)- protein-L-arginine.
-!- Arginine residues in proteins act as acceptors. -!- Free arginine, agmatine ((4-aminobutyl)guanidine), arginine methyl ester and guanidine can also do so. -!- The enzyme from some, but not all, species can also use NADP(+) as acceptor (giving rise to N(omega)-((2'-phospho-ADP)-D-ribosyl)- protein-L-arginine as the product), but more slowly. -!- Catalyzes the NAD(+)-dependent activation of EC 4.6.1.1. -!- Some bacterial enterotoxins possess similar enzymatic activities (cf. EC 2.4.2.36).

UniProtKB Entries (1)

P20974
NAR2B_RAT
Rattus norvegicus
T-cell ecto-ADP-ribosyltransferase 2

PDB Structure

PDB 1GY0
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure of the Ecto-Adp-Ribosyl Transferase Art2.2 From Rat
Mueller-Dieckmann, C., Ritter, H., Haag, F., Koch-Nolte, F., Schulz, G.E.
J.Mol.Biol.