CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.60 | 4-Layer Sandwich |
|
3.60.130 | Double-stranded beta-helix |
|
3.60.130.10 | Clavaminate synthase-like |
Domain Context
CATH Clusters
| Superfamily | Clavaminate synthase-like |
| Functional Family |
Enzyme Information
| 1.14.11.21 |
Clavaminate synthase.
based on mapping to UniProt Q05581
(1) Deoxyamidinoproclavaminate + 2-oxoglutarate + O(2) = amidinoproclavaminate + succinate + CO(2). (2) Proclavaminate + 2-oxoglutarate + O(2) = dihydroclavaminate + succinate + CO(2) + H(2)O. (3) Dihydroclavaminate + 2-oxoglutarate + O(2) = clavaminate + succinate + CO(2) + H(2)O.
-!- Catalyzes three separate oxidative reactions in the pathway for the biosythesis of the beta-lactamase inhibitor clavulanate in Streptomyces clavuligerus. -!- The first step (hydroxylation) is separated from the latter two (oxidative cyclization and desaturation) by the action of EC 3.5.3.22. -!- The three reactions are all catalyzed at the same nonheme iron site.
|
UniProtKB Entries (1)
| Q05581 |
CAS1_STRC2
Streptomyces clavuligerus ATCC 27064
Clavaminate synthase 1
|
PDB Structure
| PDB | 1GVG |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal Structure of a Clavaminate Synthase-Fe(II) -2-Oxoglutarate-Substrate-No Complex: Evidence for Metal Centered Rearrangements
FEBS Lett.
|
