CATH Domain 1gisA01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.420	Ricin (A subunit); domain 1
	3.40.420.10	Ricin (A subunit), domain 1

Domain Context

CATH Clusters

Superfamily	Ricin (A subunit), domain 1
Functional Family

Enzyme Information

3.2.2.22

rRNA N-glycosylase.

based on mapping to UniProt P09989

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

-!- Ricin A-chain and related toxins show this activity. -!- Naked rRNA is attacked more slowly than rRNA in intact ribosomes. -!- Naked rRNA from Escherichia coli is cleaved at a corresponding position.

UniProtKB Entries (1)

P09989

RIPT_TRIKI

Trichosanthes kirilowii

Ribosome-inactivating protein alpha-trichosanthin

PDB Structure

PDB	1GIS
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Substrate binding and catalysis in trichosanthin occur in different sites as revealed by the complex structures of several E85 mutants. Guo, Q., Zhou, W., Too, H.M., Li, J., Liu, Y., Bartlam, M., Dong, Y., Wong, K.B., Shaw, P.C., Rao, Z. Protein Eng.