CATH Classification

Domain Context

CATH Clusters

Superfamily NAD(P)-binding Rossmann-like Domain
Functional Family

Enzyme Information

1.1.99.28
Glucose-fructose oxidoreductase.
based on mapping to UniProt Q07982
D-glucose + D-fructose = D-gluconolactone + D-glucitol.
-!- D-mannose, D-xylose, D-galactose, 2-deoxy-D-glucose and L-arabinose will function as aldose substrates, but with low affinities. -!- The ketose substrate must be in the open-chain form. -!- The apparent affinity for fructose is low, because little of the fructose substrate is in the open-chain form. -!- Xylulose and glycerone (dihydroxyacetone) will replace fructose, but they are poor substrates. -!- The enzyme from Zymomonas mobilis contains tightly bound NADP(+).

UniProtKB Entries (1)

Q07982
GFO_ZYMMO
Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821
Glucose--fructose oxidoreductase

PDB Structure

PDB 1EVJ
External Links
Method X-RAY DIFFRACTION
Organism Zymomonas
Primary Citation
Crystal structure of a truncated mutant of glucose-fructose oxidoreductase shows that an N-terminal arm controls tetramer formation.
Lott, J.S., Halbig, D., Baker, H.M., Hardman, M.J., Sprenger, G.A., Baker, E.N.
J.Mol.Biol.