CATH Classification

Domain Context

CATH Clusters

Superfamily 2.30.30.100
Functional Family Bifunctional ligase/repressor BirA

Enzyme Information

6.3.4.15
Biotin--[biotin carboxyl-carrier protein] ligase.
based on mapping to UniProt P06709
ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine.
-!- The enzyme biotinylates a biotin carboxyl-carrier protein that is part of an acetyl-CoA carboxylase complex, enabling its subsequent carboxylation by EC 6.3.4.14. -!- The carboxyl group is eventually transferred to acetyl-CoA by EC 2.1.3.15. -!- In some organisms the carrier protein is part of EC 6.4.1.2.

UniProtKB Entries (1)

P06709
BIRA_ECOLI
Escherichia coli K-12
Bifunctional ligase/repressor BirA

PDB Structure

PDB 1BIA
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Escherichia coli biotin holoenzyme synthetase/bio repressor crystal structure delineates the biotin- and DNA-binding domains.
Wilson, K.P., Shewchuk, L.M., Brennan, R.G., Otsuka, A.J., Matthews, B.W.
Proc.Natl.Acad.Sci.USA