CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
4 | Few Secondary Structures |
|
4.10 | Irregular |
|
4.10.60 | HIV-1 Nucleocapsid Protein |
|
4.10.60.10 | Zinc finger, CCHC-type |
Domain Context
CATH Clusters
| Superfamily | Zinc finger, CCHC-type |
| Functional Family | Gag polyprotein |
Enzyme Information
| 3.1.26.13 |
Retroviral ribonuclease H.
based on mapping to UniProt P05961
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
-!- Retroviral reverse transcriptase is a multifunctional enzyme responsible for viral replication. -!- To perform this task the enzyme combines two distinct activities. -!- The polymerase domain (EC 2.7.7.49) occupies the N-terminal two- thirds of the reverse transcriptase whereas the ribonuclease H domain comprises the C-terminal remaining one-third. -!- The RNase H domain of Moloney murine leukemia virus and Human immunodeficiency virus display two metal binding sites.
|
| 3.4.23.16 |
HIV-1 retropepsin.
based on mapping to UniProt P05961
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
-!- Present in human immunodeficiency virus type 1. -!- Contributes to the maturation of the viral particle, and is a target of antiviral drugs. -!- Active enzyme is a dimer of identical 11-kDa subunits. -!- Similar enzymes occur in other retroviruses. -!- Belongs to peptidase family A2.
|
| 2.7.7.7 |
DNA-directed DNA polymerase.
based on mapping to UniProt P05961
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes DNA-template-directed extension of the 3'-end of a DNA strand by one nucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a primer which may be DNA or RNA. -!- See also EC 2.7.7.49.
|
| 2.7.7.49 |
RNA-directed DNA polymerase.
based on mapping to UniProt P05961
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
-!- Catalyzes RNA-template-directed extension of the 3'-end of a DNA strand by one deoxynucleotide at a time. -!- Cannot initiate a chain de novo. -!- Requires a RNA or DNA primer. -!- DNA can also serve as template. -!- See also EC 2.7.7.7.
|
| 2.7.7.- |
Nucleotidyltransferases.
based on mapping to UniProt P05961
|
| 3.1.-.- |
Acting on ester bonds.
based on mapping to UniProt P05961
|
| 3.1.13.2 |
Exoribonuclease H.
based on mapping to UniProt P05961
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid.
-!- This is a secondary reaction to the RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end performed by EC 3.1.26.13.
|
UniProtKB Entries (1)
| P05961 |
POL_HV1MN
HIV-1 M:B_MN
Gag-Pol polyprotein
|
PDB Structure
| PDB | 1AAF |
| External Links | |
| Method | SOLUTION NMR |
| Organism | Escherichia |
| Primary Citation |
Nucleocapsid zinc fingers detected in retroviruses: EXAFS studies of intact viruses and the solution-state structure of the nucleocapsid protein from HIV-1.
Protein Sci.
|