CATH Domain 5dwsC01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.20	Single Sheet
	2.20.70	Ubiquitin Ligase Nedd4; Chain: W;
	2.20.70.10

Domain Context

CATH Clusters

Superfamily	2.20.70.10
Functional Family	E3 ubiquitin-protein ligase

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q96J02

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q96J02

ITCH_HUMAN

Homo sapiens

E3 ubiquitin-protein ligase Itchy homolog

PDB Structure

PDB	5DWS
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal Structure of ITCH WW3 domain in complex with TXNIP peptide Liu, Y., Tempel, W., Dong, A., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Min, J. to be published