CATH Domain 3p3nA01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.120	Jelly Rolls
	2.60.120.10	Jelly Rolls

Domain Context

CATH Clusters

Superfamily	Jelly Rolls
Functional Family	Hypoxia-inducible factor 1-alpha inhibitor

Enzyme Information

1.14.11.n4

Ankyrin-repeat-histidine dioxagenase.

based on mapping to UniProt Q9NWT6

(1) [Ankyrin-repeat domain protein]-L-histidine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-histidine + succinate + CO(2). (2) [Ankyrin-repeat domain protein]-L-asparagine + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-asparagine + succinate + CO(2). (3) [Ankyrin-repeat domain protein]-L-aspartate + 2-oxoglutarate + O(2) = [ankyrin-repeat domain protein]-(3S)-3-hydroxy-L-aspartate + succinate + CO(2).

1.14.11.30

Hypoxia-inducible factor-asparagine dioxygenase.

based on mapping to UniProt Q9NWT6

Hypoxia-inducible factor-L-asparagine + 2-oxoglutarate + O(2) = hypoxia- inducible factor-(3S)-3-hydroxy-L-asparagine + succinate + CO(2).

-!- Contains iron, and requires ascorbate. -!- Catalyzes hydroxylation of an asparagine in the C-terminal transcriptional activation domain of HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which reduces its interaction with the transcriptional coactivator protein p300. -!- The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.

UniProtKB Entries (2)

Q01705	NOTC1_MOUSE Mus musculus Neurogenic locus notch homolog protein 1
Q9NWT6	HIF1N_HUMAN Homo sapiens Hypoxia-inducible factor 1-alpha inhibitor

PDB Structure

PDB	3P3N
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Asparaginyl hydroxylation of the Notch ankyrin repeat domain by factor inhibiting hypoxia-inducible factor Coleman, M.L., Mcdonough, M.A., Hewitson, K.S., Coles, C., Mecinovic, J., Edelmann, M., Cook, K.M., Cockman, M.E., Lancaster, D.E., Kessler, B.M., Oldham, N.J., Ratcliffe, P.J., Schofield, C.J. J.Biol.Chem.