CATH Domain 2f2hF02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.20	Alpha-Beta Barrel
	3.20.20	TIM Barrel
	3.20.20.80	Glycosidases

Domain Context

CATH Clusters

Superfamily	Glycosidases
Functional Family	Alpha-xylosidase YicI

Enzyme Information

3.2.1.177

Alpha-D-xyloside xylohydrolase.

based on mapping to UniProt P31434

Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose.

-!- The enzyme catalyzes hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide. -!- Representative alpha-xylosidases from glycoside hydrolase family 31 utilize a two-step (double-displacement) mechanism involving a covalent glycosyl-enzyme intermediate, and retain the anomeric configuration of the product.

UniProtKB Entries (1)

P31434

XYLS_ECOLI

Escherichia coli K-12

Alpha-xylosidase

PDB Structure

PDB	2F2H
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Expanding the Thioglycoligase Strategy to the Synthesis of alpha-linked Thioglycosides Allows Structural Investigation of the Parent Enzyme/Substrate Complex Kim, Y.-W., Lovering, A.L., Chen, H., Kantner, T., McIntosh, L.P., Strynadka, N.C.J., Withers, S.G. J.Am.Chem.Soc.