CATH Classification

Domain Context

CATH Clusters

Superfamily Hect, E3 ligase catalytic domains
Functional Family E3 ubiquitin-protein ligase

Enzyme Information

2.3.2.26
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt P39940
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

P39940
RSP5_YEAST
Saccharomyces cerevisiae S288C
E3 ubiquitin-protein ligase RSP5

PDB Structure

PDB 3OLM
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structure and function of a HECT domain ubiquitin-binding site.
Kim, H.C., Steffen, A.M., Oldham, M.L., Chen, J., Huibregtse, J.M.
Embo Rep.
CATH-Gene3D is a Global Biodata Core Resource Learn more...