CATH Domain 2lmcB00

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.40	Beta Barrel
	2.40.50	OB fold (Dihydrolipoamide Acetyltransferase, E2P)
	2.40.50.100	RNA polymerase II/Efflux pump adaptor protein, barrel-sandwich hybrid domain

Domain Context

CATH Clusters

Superfamily	2.40.50.100
Functional Family	DNA-directed RNA polymerase subunit beta

Enzyme Information

2.7.7.6

DNA-directed RNA polymerase.

based on mapping to UniProt P0A8T7

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

-!- Catalyzes DNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. -!- Can initiate a chain de novo. -!- In eukaryotes three forms of the enzyme have been distinguished on the basis of sensitivity of alpha-amanitin, and the type of RNA synthesized. -!- See also EC 2.7.7.19 and EC 2.7.7.48.

UniProtKB Entries (2)

P0A8T7	RPOC_ECOLI Escherichia coli K-12 DNA-directed RNA polymerase subunit beta'
P03704	VRPI_BPT7 Enterobacteria phage T7 Bacterial RNA polymerase inhibitor

PDB Structure

PDB	2LMC
External Links	PDBSum Proteopedia
Method	SOLUTION NMR
Organism
Primary Citation	Structural and Mechanistic Basis for the Inhibition of Escherichia coli RNA Polymerase by T7 Gp2. James, E., Liu, M., Sheppard, C., Mekler, V., Camara, B., Liu, B., Simpson, P., Cota, E., Severinov, K., Matthews, S., Wigneshweraraj, S. Mol.Cell