CATH Classification

Domain Context

CATH Clusters

Superfamily Cyclic phosphodiesterase
Functional Family RNA 2',3'-cyclic phosphodiesterase

Enzyme Information

3.1.4.58
RNA 2',3'-cyclic 3'-phosphodiesterase.
based on mapping to UniProt O57823
(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)- 2'-phosphate.
-!- The enzyme hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester. -!- In vitro the enzyme can also ligate tRNA molecules with 2',3'-cyclic phosphate to tRNA with 5'-hydroxyl termini, forming a 2'-5' phosphodiester linkage. -!- However, the ligase activity is unlikely to be relevant in vivo.

UniProtKB Entries (1)

O57823
THPR_PYRHO
Pyrococcus horikoshii OT3
RNA 2',3'-cyclic phosphodiesterase

PDB Structure

PDB 1VGJ
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The structure of Pyrococcus horikoshii 2'-5' RNA ligase at 1.94 A resolution reveals a possible open form with a wider active-site cleft
Gao, Y.G., Yao, M., Okada, A., Tanaka, I.
ACTA CRYSTALLOGR.,SECT.F