CATH Classification
Level | CATH Code | Description |
---|---|---|
3 | Alpha Beta | |
3.30 | 2-Layer Sandwich | |
3.30.2160 | Hect, E3 ligase catalytic domain | |
3.30.2160.10 | Hect, E3 ligase catalytic domain |
Domain Context
CATH Clusters
Superfamily | Hect, E3 ligase catalytic domain |
Functional Family | probable E3 ubiquitin-protein ligase HERC4 isoform X1 |
Enzyme Information
2.3.2.26 |
HECT-type E3 ubiquitin transferase.
based on mapping to UniProt Q05086
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.
-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.
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UniProtKB Entries (1)
Q05086 |
UBE3A_HUMAN
Homo sapiens
Ubiquitin-protein ligase E3A
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PDB Structure
PDB | 1D5F |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | Escherichia |
Primary Citation |
Structure of an E6AP-UbcH7 complex: insights into ubiquitination by the E2-E3 enzyme cascade.
Science
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