CATH Domain 4uwdA00

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.120	Jelly Rolls
	2.60.120.620	q2cbj1_9rhob like domain

Domain Context

CATH Clusters

Superfamily	q2cbj1_9rhob like domain
Functional Family	Egl nine homolog 1

Enzyme Information

1.14.11.29

Hypoxia-inducible factor-proline dioxygenase.

based on mapping to UniProt Q9GZT9

Hypoxia-inducible factor-L-proline + 2-oxoglutarate + O(2) = hypoxia- inducible factor-trans-4-hydroxy-L-proline + succinate + CO(2).

-!- Contains iron, and requires ascorbate. -!- Specifically hydroxylates a proline residue in HIF-alpha, the alpha subunit of the transcriptional regulator HIF (hypoxia-inducible factor), which targets HIF for proteasomal destruction. -!- The requirement of oxygen for the hydroxylation reaction enables animals to respond to hypoxia.

UniProtKB Entries (1)

Q9GZT9

EGLN1_HUMAN

Homo sapiens

Egl nine homolog 1

PDB Structure

PDB	4UWD
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Investigating the Contribution of the Active Site Environment to the Slow Reaction of Hypoxia-Inducible Factor Prolyl Hydroxylase Domain 2 with Oxygen. Tarhonskaya, H., Chowdhury, R., Leung, I.K.H., Loik, N.D., Mccullagh, J.S.O., Claridge, T.D.W., Schofield, C.J., Flashman, E. Biochem.J.