CATH Domain 6mujA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1580	paralog of FGE (formylglycine-generating enzyme)
	3.90.1580.10	paralog of FGE (formylglycine-generating enzyme)

Domain Context

CATH Clusters

Superfamily	paralog of FGE (formylglycine-generating enzyme)
Functional Family	Formylglycine-generating enzyme family protein

Enzyme Information

1.8.3.7

Formylglycine-generating enzyme.

based on mapping to UniProt Q9F3C7

A [sulfatase]-L-cysteine + O(2) + 2 a thiol = a [sulfatase]-3-oxo-L- alanine + hydrogen sulfide + a disulfide + H(2)O.

-!- The enzyme, which is found in both prokaryotes and eukaryotes, catalyzes a modification of a conserved L-cysteine residue in the active site of sulfatases, generating a unique 3-oxo-L-alanine residue that is essential for sulfatase activity. -!- The exact nature of the thiol involved is still not clear - dithiothreitol and cysteamine are the most efficiently used thiols in vitro. -!- Glutathione alo acts in vitro, but it is not known whether it is used in vivo.

UniProtKB Entries (1)

Q9F3C7

FGE_STRCO

Streptomyces coelicolor A3(2)

Formylglycine-generating enzyme

PDB Structure

PDB	6MUJ
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Formylglycine-generating enzyme binds substrate directly at a mononuclear Cu(I) center to initiate O2activation. Appel, M.J., Meier, K.K., Lafrance-Vanasse, J., Lim, H., Tsai, C.L., Hedman, B., Hodgson, K.O., Tainer, J.A., Solomon, E.I., Bertozzi, C.R. Proc. Natl. Acad. Sci. U.S.A.

Network disruptions