CATH Classification

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family Ubiquitin-conjugating enzyme E2 L3

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P68036
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

UniProtKB Entries (2)

P68036
UB2L3_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 L3
P69326
UBIQ_WHEAT
Triticum aestivum
Ubiquitin

PDB Structure

PDB 5TTE
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.
Yuan, L., Lv, Z., Atkison, J.H., Olsen, S.K.
Nat Commun