CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.20 | Up-down Bundle |
|
1.20.120 | Four Helix Bundle (Hemerythrin (Met), subunit A) |
|
1.20.120.1750 |
Domain Context
CATH Clusters
| Superfamily | 1.20.120.1750 |
| Functional Family | RBR-type E3 ubiquitin transferase |
Enzyme Information
| 2.3.2.31 |
RBR-type E3 ubiquitin transferase.
based on mapping to UniProt Q9Y4X5
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.
-!- RBR-type E3 ubiquitin transferases have two RING fingers separated by an internal motif (IBR, for In Between RING). -!- The enzyme interacts with the CRL (Cullin-RING ubiquitin Ligase) complexes formed by certain RING-type E3 ubiquitin transferase (see EC 2.3.2.27), which include a neddylated cullin scaffold protein and a substrate recognition module. -!- The RING1 domain binds an EC 2.3.2.23, and transfers the ubiquitin that is bound to it to an internal cysteine residue in the RING2 domain, followed by the transfer of the ubiquitin from RING2 to the substrate. -!- Once the substrate has been ubiquitinated by the RBR-type ligase, it can be ubiqutylated further using ubiquitin carried directly on E2 enzymes, in a reaction catalyzed by EC 2.3.2.27. -!- Activity of the RBR-type enzyme is dependent on neddylation of the cullin protein in the CRL complex. -!- Cf. EC 2.3.2.26, EC 2.3.2.27, and EC 2.3.2.32.
|
UniProtKB Entries (2)
| P68036 |
UB2L3_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 L3
|
| P69326 |
UBIQ_WHEAT
Triticum aestivum
Ubiquitin
|
PDB Structure
| PDB | 5TTE |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structural insights into the mechanism and E2 specificity of the RBR E3 ubiquitin ligase HHARI.
Nat Commun
|
