CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.1820 | alpha/beta hydrolase |
Domain Context
CATH Clusters
| Superfamily | alpha/beta hydrolase |
| Functional Family | Pimeloyl-[acyl-carrier protein] methyl ester esterase |
Enzyme Information
| 3.1.1.85 |
Pimeloyl-[acyl-carrier protein] methyl ester esterase.
based on mapping to UniProt Q83PW0
Pimeloyl-[acyl-carrier protein] methyl ester + H(2)O = pimeloyl-[acyl- carrier protein] + methanol.
-!- Involved in biotin biosynthesis in Gram-negative bacteria. -!- The enzyme exhibits carboxylesterase activity, particularly toward substrates with short acyl chains. -!- Even though the enzyme can interact with coenzyme A thioesters, the in vivo role of the enzyme is to hydrolyze the methyl ester of pimeloyl-[acyl carrier protein], terminating the part of the biotin biosynthesis pathway that is catalyzed by the fatty acid elongation enzymes.
|
UniProtKB Entries (2)
| Q83PW0 |
BIOH_SHIFL
Shigella flexneri
Pimeloyl-[acyl-carrier protein] methyl ester esterase
|
| Q0T5U2 |
ACP_SHIF8
Shigella flexneri 5 str. 8401
Acyl carrier protein
|
PDB Structure
| PDB | 4ETW |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structure of the enzyme-acyl carrier protein (ACP) substrate gatekeeper complex required for biotin synthesis.
Proc.Natl.Acad.Sci.USA
|