CATH Domain 4dm9A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.532	Ubiquitin C-terminal Hydrolase UCH-l3
	3.40.532.10	Peptidase C12, ubiquitin carboxyl-terminal hydrolase

Domain Context

CATH Clusters

Superfamily	Peptidase C12, ubiquitin carboxyl-terminal hydrolase
Functional Family	Ubiquitin carboxyl-terminal hydrolase

Enzyme Information

3.4.19.12

Ubiquitinyl hydrolase 1.

based on mapping to UniProt P09936

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P09936

UCHL1_HUMAN

Homo sapiens

Ubiquitin carboxyl-terminal hydrolase isozyme L1

PDB Structure

PDB	4DM9
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE(OMe)-FMK). Davies, C.W., Chaney, J., Korbel, G., Ringe, D., Petsko, G.A., Ploegh, H., Das, C. Bioorg.Med.Chem.Lett.