CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.20 | Alpha-Beta Barrel |
|
3.20.20 | TIM Barrel |
|
3.20.20.70 | Aldolase class I |
Domain Context
CATH Clusters
| Superfamily | Aldolase class I |
| Functional Family |
Enzyme Information
| 4.1.3.44 |
tRNA 4-demethylwyosine synthase (AdoMet-dependent).
based on mapping to UniProt O59412
N(1)-methylguanine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + L-methionine + 5'-deoxyadenosine + CO(2) + H(2)O.
-!- This enzyme, which is a member of the superfamily of S-adenosyl-L- methionine-dependent radical (radical AdoMet) enzymes, binds two [4Fe-4S] clusters. -!- Carbons C(2) and C(3) from pyruvate are incorporated into 4-demethylwyosine. -!- The enzyme is found in eukaryotes where it is part of the pathway for wybutosine synthesis, and in archaea, where it is involved in the biosynthesis of archaeal wye bases, such as wyosine, isowyosine, and methylwyosine.
|
UniProtKB Entries (1)
| O59412 |
TYW1_PYRHO
Pyrococcus horikoshii OT3
S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase
|
PDB Structure
| PDB | 2YX0 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
Structure of an archaeal TYW1, the enzyme catalyzing the second step of wye-base biosynthesis
Acta Crystallogr.,Sect.D
|
