CATH Domain 2jk7A00

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.1170	Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
	1.10.1170.10	Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A

Domain Context

CATH Clusters

Superfamily	Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
Functional Family	E3 ubiquitin-protein ligase XIAP

Enzyme Information

2.3.2.27

RING-type E3 ubiquitin transferase.

based on mapping to UniProt P98170

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- RING E3 ubiquitin transferases serve as mediators bringing the ubiquitin-charged E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) and an acceptor protein together to enable the direct transfer of ubiquitin through the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon-amino group of an L-lysine residue of the acceptor protein. -!- Unlike EC 2.3.2.26 the RING-E3 domain does not form a catalytic thioester intermediate with ubiquitin. -!- Many members of the RING-type E3 ubiquitin transferase family are not able to bind a substrate directly, and form a complex with a cullin scaffold protein and a substrate recognition module (the complexes are named CRL for Cullin-RING-Ligase). -!- In these complexes, the RING-type E3 ubiquitin transferase provides an additional function, mediating the transfer of a NEDD8 protein from a dedicated E2 carrier to the cullin protein (see EC 2.3.2.32). -!- Cf. EC 2.3.2.31.

UniProtKB Entries (1)

P98170

XIAP_HUMAN

Homo sapiens

E3 ubiquitin-protein ligase XIAP

PDB Structure

PDB	2JK7
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structure-Based Design, Synthesis, Evaluation, and Crystallographic Studies of Conformationally Constrained Smac Mimetics as Inhibitors of the X-Linked Inhibitor of Apoptosis Protein (Xiap). Sun, H., Stuckey, J.A., Nikolovska-Coleska, Z., Qin, D., Meagher, J.L., Qiu, S., Lu, J., Yang, C., Saito, N.G., Wang, S. J.Med.Chem.