CATH Classification

Domain Context

CATH Clusters

Superfamily Endonuclease/exonuclease/phosphatase
Functional Family

Enzyme Information

3.1.-.-
Acting on ester bonds.
based on mapping to UniProt P27695
4.2.99.18
DNA-(apurinic or apyrimidinic site) lyase.
based on mapping to UniProt P27695
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
-!- 'Nicking' of the phosphodiester bond is due to a lyase-type reaction, not hydrolysis. -!- This group of enzymes was previously listed as endonucleases, under the number EC 3.1.25.2.

UniProtKB Entries (1)

P27695
APEX1_HUMAN
Homo sapiens
DNA-(apurinic or apyrimidinic site) lyase

PDB Structure

PDB 2ISI
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Crystal structure of hApe1 in a new crystal form with a bound ligand: implications on catalytic mechanism and its inhibition
Agarwal, R., Naidu, M.D.
To be Published