CATH Domain 2c5sA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.620	HUPs

Domain Context

CATH Clusters

Superfamily	HUPs
Functional Family	Probable tRNA sulfurtransferase

Enzyme Information

2.8.1.4

tRNA uracil 4-sulfurtransferase.

based on mapping to UniProt Q81KU0

ATP + [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + uracil in tRNA + 2 reduced ferredoxin [iron-sulfur] cluster = AMP + diphosphate + 4-thiouracil in tRNA + [ThiI sulfur-carrier protein]-L-cysteine + 2 oxidized ferredoxin [iron-sulfur] cluster.

-!- The enzyme, found in bacteria and archaea, is activated by EC 2.8.1.7, which transfers a sulfur atom to an internal L-cysteine residue, forming a cysteine persulfide. -!- The activated enzyme then transfers the sulfur to a uridine in a tRNA chain in a reaction that requires ATP. -!- The enzyme from the bacterium Escherichia coli forms 4-thiouridine only at position 8 of tRNA. -!- The enzyme also participates in the biosynthesis of the thiazole moiety of thiamine, but different domains are involved in the two processes.

UniProtKB Entries (1)

Q81KU0

THII_BACAN

Bacillus anthracis

Probable tRNA sulfurtransferase

PDB Structure

PDB	2C5S
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal structure of Bacillus anthracis ThiI, a tRNA-modifying enzyme containing the predicted RNA-binding THUMP domain. Waterman, D.G., Ortiz-Lombardia, M., Fogg, M.J., Koonin, E.V., Antson, A.A. J.Mol.Biol.

Network disruptions