CATH Classification
Level | CATH Code | Description |
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3 | Alpha Beta |
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3.30 | 2-Layer Sandwich |
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3.30.1360 | Gyrase A; domain 2 |
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3.30.1360.170 |
Domain Context
CATH Clusters
Superfamily | 3.30.1360.170 |
Functional Family |
Enzyme Information
2.1.1.148 |
Thymidylate synthase (FAD).
based on mapping to UniProt Q9WYT0
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.
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UniProtKB Entries (1)
Q9WYT0 |
THYX_THEMA
Thermotoga maritima MSB8
Flavin-dependent thymidylate synthase
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PDB Structure
PDB | 1KQ4 |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | Escherichia |
Primary Citation |
Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline
Proc.Natl.Acad.Sci.USA
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