CATH Domain 3iweA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.630	Aminopeptidase
	3.40.630.170

Domain Context

CATH Clusters

Superfamily	3.40.630.170
Functional Family	Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97

Glycylpeptide N-tetradecanoyltransferase.

based on mapping to UniProt P30419

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].

-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

P30419

NMT1_HUMAN

Homo sapiens

Glycylpeptide N-tetradecanoyltransferase 1

PDB Structure

PDB	3IWE
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal Structure of human type-I N-myristoyltransferase with bound myristoyl-CoA and inhibitor DDD85646 Qiu, W., Hutchinson, A., Wernimont, A., Lin, Y.-H., Kania, A., Ravichandran, M., Kozieradzki, I., Cossar, D., Schapira, M., Arrowsmith, C.H., Bountra, C., Weigelt, J., Edwards, A.M., Wyatt, P.G., Ferguson, M.A.J., Frearson, J.A., Brand, S.Y., Robinson, D.A., Bochkarev, A., Hui, R. To be Published